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🧵 New pre-print out from my time at @mib-wur.bsky.social showing how the titration of the initiator protein DnaA on the chromosome of E. coli can generate stable DNA replication @hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social doi.org/10.64898/202...
1mo
Lorenzo Olivi
Controlling the expression of an always-active DnaA mutant and using the native titration power of the chromosome, we show strengths and limits of titration-based control of replication. What we saw tells us about the evolution of E. coli, but also informs design for such systems in synthetic cells!
1mo
Lorenzo Olivi