🧵 New pre-print out from my time at @mib-wur.bsky.social
showing how the titration of the initiator protein DnaA on the chromosome of E. coli can generate stable DNA replication @hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social
doi.org/10.64898/202...
Lorenzo Olivi
Controlling the expression of an always-active DnaA mutant and using the native titration power of the chromosome, we show strengths and limits of titration-based control of replication. What we saw tells us about the evolution of E. coli, but also informs design for such systems in synthetic cells!