A day in the life of a growing hiPSC spheroid expressing endogenously mStayGold-tagged histone 3.3 for #FluorescenceFriday and the #Snouty aficionados. 28 hours (and >50,000 exposures) later and they are still going! Home-brewed volume projection.
We’re hiring a scientist to work on microscopy at Calico! If you’re excited about developing and applying cutting edge microscopy to a variety of biological questions, we’d love to hear from you.
Find more information and apply here: www.calicolabs.com/careers/?gh_...
Altair Direct View OPM Preprint!
We built a simplified direct-view oblique-plane microscope for imaging big, optically accessible specimens with a single-objective light-sheet geometry. Delivers...
• ~5.4 mm field of view
• ~2.6 μm, ~3.4 μm, and ~17.8 μm resolution in X/Y/Z
What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~
www.biorxiv.org/content/10.6...
We present:
Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution
www.biorxiv.org/content/10.6...
Hello #world, meet 1,000× Expansion Microscopy.
A small gel would grow to the size of an Olympic swimming pool, while amino-acid-scale distances become visible with ordinary light microscopy.
Led by Helena Hu from @eboyden3.bsky.social's lab, in collab with us.
Story: www.biorxiv.org/content/10.6...
#HappyMicroscopyMonday #microscopycommunity - Join the BINA Builders for a panel and discussion on the shift from proprietary systems to community-driven innovation. Thursday, May 21!
Learn more: buff.ly/pMShl9t
Video
Video
We invented “triplet tumbling microscopy” (TTM) to track protein-protein interactions in living cells!
Tag your protein with GFP and see it interact with *untagged* binding partners
www.biorxiv.org/content/10.6...
