We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better.
Preprint: www.biorxiv.org/content/10.6...
Code: github.com/warpem/miss-...
🧶 Thread:
Super cool cryo-ET work from my colleague Xiaohan resolving a ton of ribosome assembly intermediates in the nuclei of human cells!
And also a similar microscope from Falckenhayn et al. in the Kaufmann group last week:
www.biorxiv.org/content/10.6...
Congratulations, Julia Mahamid!
Julia Mahamid receives the @dfg.de Leibniz Prize for her work on structural cell biology!
#LeibnizPreis
Video
People interested in the VPP aspects should also see a recent paper (from the people at Biohub) showing that tilt-series alignments can be improved with phase plate usage.
journals.iucr.org/m/issues/202...
Really excellent work by the bluesky-less Marie and Sara +coauthors in our lab @embl.org! Check it out for cool combinations of light microscopy and #cryoET, and if you're interested in how yeasts reorganize structurally in response to stressors. #teamtomo
See also our preprint about the microscope itself, which went up earlier this afternoon!
www.biorxiv.org/content/10.6...
We @embl.org wrote a short preprint about optimizing for maximum completeness in #cryoet analysis. We looked at template matching, VPP imaging, and tilt-series refinement parameters, and why averaging ribosomes helps even if you aren’t interested in their biology 🏭
www.biorxiv.org/content/10.6...
Big news for correlative cryogenic SMLM/ET! With my colleague Soheil, we (Mahamid and @jonasries.bsky.social labs) indicate lamella-specific illumination can allow 500-1500x higher laser intensities, opening massive new possibilities for correlative imaging #teamtomo
www.biorxiv.org/content/10.6...
Dimitry Tegunov
joe dobbs
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️
www.biorxiv.org/content/10.1...
#teamtomo
joe dobbs
Video
EMBL
www.biorxiv.org
joe dobbs
joe dobbs
joe dobbs
joe dobbs
joe dobbs
joe dobbs
In-cell structures visualize human pre-ribosome assembly in the nucleolus https://www.biorxiv.org/content/10.64898/2026.06.01.729245v1
