Side note: One often thinks they're not recovering low-abundance taxa because they're low-abundance. But with Illumina, it's also likely because they're low-GC and Illumina has strong GC bias. You can fix this with wetlab intervention like this, or using ONT instead, which doesn't have a GC-bias.🧵
Steven Robbins
Genomic recovery from rare terrestrial microbes enabled by DNA-based GC-fractionation | ISME Communications | Oxford Academic https://academic.oup.com/ismecommun/advance-article/doi/10.1093/ismeco/ycaf152/8246624?rss=1&login=false
Abstract. Genome reconstruction from metagenomic samples has dramatically increased our understanding of uncultivated lineages of life. However, untargeted
