Additional crucial contributions were provided by @andrew-petti.bsky.social, Xuntao Sheena Xhao, Tingting Jiang, and several other colleagues.
11/15
Now with SharedIt link:
rdcu.be/ff6Zf
📰 Hot off the press from the Xue & Sontheimer labs 🧬: Prime assembly enables precise genomic insertions up to 11 kb in cells, providing a universal strategy to correct many pathogenic mutations within a gene. Read it @nature.com:
buff.ly/dYn0NjS
@UMassChan.bsky.social @eriksontheimer.bsky.social
Bin now has a lab of his own (www.binliucrispr.org) at @osuwexmed.bsky.social.
10/15
Similar large-insertion strategies were also reported last week by Hao Yin and Ying Zhang from Wuhan University:
www.nature.com/articles/s41...
13/15
To re-cap: prime assembly enables site-specific, multi-kilobase insertions and over-writes in mammalian cells, including via fully or predominantly single-stranded DNA donors – all without DSBs, HDR, recombinases, integrases, transposases, or active cell cycling.
14/15
Comparable work (forthcoming) was done independently by @sebastien-levesque.bsky.social, @danielevanbauer.bsky.social and colleagues. Earlier @biorxivpreprint.bsky.social versions from June 2025 are here:
doi.org/10.1101/2025...
doi.org/10.1101/2025...
12/15
This work was a great collaboration with the lab of my @rti-umasschan.bsky.social colleague Wen Xue (@xueumass.bsky.social). The project was spearheaded by @binliucrispr.bsky.social, a postdoc jointly shared between the two labs.
9/15
We hope you check out our paper at www.nature.com/articles/s41...!
15/15
