Find the CrAAVe-seq plasmid pAP215 here: www.addgene.org/217635/
Exciting work, thanks for sharing!
Plasmid pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2 from Dr. Martin Kampmann's lab contains the insert mU6-sgRNA, Handle sequence, EF1a-mTagBFP2 and is published in Nat Neurosci. 2025 Aug 22. doi: 10.1...
Excited that the paper presenting our mouse brain in vivo CRISPR screening platform is out today in @natneuro.nature.com!
Great team effort, led by Biswa Ramani and @ivlrose.bsky.social in the Kampmann lab.
www.nature.com/articles/s41...
The authors developed an adeno-associated virus-based high-throughput in vivo CRISPR screening platform for endogenous mouse brain cell types. Using this platform, they define genes and pathways essen...
