PhD candidate in the Beach and Oakes Labs at Loyola University Chicago. I like microscopes. I like neurons. I like bread.
Joey Tidei
Cell body getting taken for a ride in this early stage neuron. Microtubules🔵 and Actin🟣 labeled and imaged for 16hr on a Zeiss LSM880. Happy #FluorescenceFriday
Just me again with more neurons. Happy #FluorescenceFriday. Have a great weekend.
🟢FastAct™ goes green! 🟢🔬
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪
Microtubules 🟢 protruding into the dynamic actin 🟣 network of a neuronal growth cone in DIV2 hippocampal neurons. @spirochrome.com SiR-Tubulin and SPY-FastActX working wonders on this #FluorescenceFriday
Up close and personal with a branching neurite in a DIV2 hippocampal neuron expressing FTractin-GFP. This was imaged every 6 seconds over 10 minutes on the trusty @zeiss-microscopy.bsky.social LSM880. Happy #FluorescenceFriday
I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy
So excited to share the preprint with the bulk of my PhD work in the Beach @myosincity.bsky.social and Oakes @pwoakes.bsky.social Labs at Loyola University Chicago! We investigate the structure, recruitment, and function of septin networks around the nucleus. A thread:
doi.org/10.64898/202...
It's funky axons time 🔬 🧪
Our new preprint is out! Led by @stefanosala89.bsky.social sky.social we investigate the difference between molecular tension sensing and global tension on the focal adhesion. Follow along for a thread of our findings:
www.biorxiv.org/content/10.1...
Two new 🔥pre-prints 🔥 TODAY by us & @pwoakes.bsky.social @myosincity.bsky.social
change the way we think about the septin cytoskeleton
Septins buffer mechanical stress on actin and membranes!
Septins are like intermediate filaments
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
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Joey Tidei
Joey Tidei
Joey Tidei
Spirochrome
Joey Tidei
Joey Tidei
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Maggie Utgaard
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Video
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Christophe 🔬 Leterrier
Mechanotransduction is fundamental to cell signaling and depends on force-sensitive adhesion proteins. How these proteins differentiate and integrate their responses to tension remains an open questio...
