Cell, Developmental and Vascular Biologist. Laboratory for Vascular Morphogenesis. RIKEN Center for Biosystems Dynamics Research.
Li-Kun Phng
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By coupling Piezo1-dependent Ca signalling to VRAC activity and aquaporin-mediated water efflux, HECs resolve the mechanical conflict between actomyosin-driven rounding and membrane integrity.
Li-Kun Phng
Thank you @navbo.bsky.social for featuring @phnglab.bsky.social as Lab of the Month in May! www.navbo.org/members-labs/
We propose that, in addition to transcriptional programs, successful hematopoietic stem and progenitor cell (HSPC) extrusion from the dorsal aorta requires modulation of intracellular pressure through coordinated ion flux and water transport.
Disruption of aquaporin-mediated water efflux leads to excessive HEC swelling and rupture, as shown here in this movie, ultimately reducing HSPC production. (Green cells are gata2b+ cells.)
Looking forward to participating in this top-notch meeting!
Latest preprint from @phnglab.bsky.social @riken-bdr.bsky.social ! We show cellular hydraulics as a determinant of hemogenic endothelial cell (HEC) survival during endothelial-to-hematopoietic (EHT) transition.
www.biorxiv.org/cgi/content/...
#mechanobiology #zebrafish #endothelialcells
Do also check out amazing videos of cortical actin dynamics in #zebrafish #endothelialcells such as this one! @riken-bdr.bsky.social
Here's the link to the original article if you haven't checked it out already! www.nature.com/articles/s41...
Happy to share a write-up of our recent publication @natcomms.nature.com demonstrating
how circumferential actomyosin-driven endothelial cell contractions constrict vessel diameter, providing insights into mechanisms of normal vessel remodelling and CCM.
www.bdr.riken.jp/en/news/rese...
🚨 How do mechanical forces shape a developing organ?
Our new #ScienceAdvances @science.org study, led by the amazing @cvagenapantoula.bsky.social, reveals a Piezo1-driven hydraulic mechanism, where mechanical cues control cell volume to guide cardiac formation ❤️🐟
🔗 www.science.org/doi/10.1126/...
