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interested in resistance pathway dynamics in plant immunity @mpipz.bsky.social
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TIRs are key regulators of plant immunity, but the role of conserved TIR-only proteins has remained unclear. Here, we compare TIR-only proteins from Arabidopsis and barley and show that they share a conserved role in plant immunity. www.biorxiv.org/content/10.6...
1d
Toll/interleukin-1/resistance (TIR) domain NADase enzymes signal in plant immunity by producing ribosylated nucleotide second messengers which activate EDS1 dimer-helper NLR pathways to restrict patho...www.biorxiv.org
Conserved TIR-only proteins drive transcriptional defense and basal immunity in dicot and monocot plants
@hennilaessle.bsky.social @federicalocci.bsky.social Many thanks to all our collaborators from @mpipz.bsky.social and beyond for their support, discussions, and contributions to this work
1d
Together, our results suggest that conserved TIR-only proteins represent an important component of plant immune signaling that promotes basal defense in both monocots and dicots.
2/7 We previously identified a distinct family of conserved TIR-only proteins present across flowering plants, including monocots that lack canonical TNL immune receptors. Whether these proteins perform similar immune functions in monocots and dicots remained unresolved.
4/7 Using CRISPR-generated tir-only mutants in Arabidopsis and barley, together with barley eds1-pad4 mutants, we found that loss of conserved TIR-only proteins increases susceptibility to virulent filamentous pathogens in both species.
7/7 Transcriptome analysis in barley linked conserved TIR-only and EDS1-PAD4 signaling to immune-associated transcriptional responses and calcium-related pathways.
1d
6/7 We further show that conserved TIR-only proteins contribute to PAMP-triggered immunity. Mutants displayed impaired induction of defense marker genes following flg22 treatment, while early responses such as ROS production and MAPK activation remained intact.
5/7 In contrast, conserved TIR-only proteins were not required for resistance to avirulent pathogens, indicating a role in basal immunity rather than classical NLR-mediated effector-triggered immunity.
1d