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And here is another direct link to the preprint. Enjoy! www.biorxiv.org/content/10.6...
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www.biorxiv.org
Agrobacterium-mediated transformation is the dominant method for plant transgenesis, yet it frequently produces multi-copy, structurally complex T-DNA insertions associated with transgene silencing, u...
Rational design of T-DNA vectors enables predictable, single-copy integration in Arabidopsis thaliana
Mo Khalil
๐ŸŒฑ NEW PREPRINT & SPECIAL day! We unveil an advance for plant science, engineering, and synbio in first (of many) Khalil <> Gehring collabs, led by the one-and-only @willshaw.bsky.social. Thread below on principles + design of a new, single-integrating T-DNA vector for precision plant xformation! ๐Ÿ‘‡
4d
Mo Khalil
New preprint out ๐ŸŒฑ We present a new T-DNA vector system for Arabidopsis that supports clean, genomically mapped, single-copy T-DNA insertion with predictable cell-type/conditional gene expression. @mokhalil.bsky.social + Gehring labs biorxiv.org/content/10.6... ๐Ÿงต1/16
4d
Agrobacterium-mediated transformation is the dominant method for plant transgenesis, yet it frequently produces multi-copy, structurally complex T-DNA insertions associated with transgene silencing, u...
biorxiv.org
Rational design of T-DNA vectors enables predictable, single-copy integration in Arabidopsis thaliana
Will Shaw