This reassignment first emerged from our broader bacterial genetic code analysis in our recent Fakih et al. NAR 2026 (doi.org/10.1093/nar/...) and here we provide a detailed characterization of its phylogenetic, codon-usage, tRNA, and aminoacylation context.
Abstract. Recently, a tRNATrpCCA with a 4-base-pair (bp) anticodon stem (AS) was shown to efficiently recognize a near-cognate UGA codon in unicellular euk