Inlay

Principal Investigator | Northwestern University Microbiology-Immunology | #RNA #ribosome #antibiotic resistance #AMR #MRSA | hails from 🇲🇾 by way of 🇹🇼 |Views are my own. 🧪 🧬🔬💉

🔽 (the first) ribosomal E-site targeting antibacterial; producer avoids self-toxicity by rRNA methylation. #Ribosome #AMR

The NIH has been holding back grants from funds Congress has already approved. www.wsj.com/opinion/get-...

Shedding light on YfhS and YjlC: novel effectors of the NADH dehydrogenase activity of the electron transport chain in Bacillus subtilis bioRxiv from @eswaralab.bsky.social www.biorxiv.org/content/10.6...

Great review on glutmate metabolism. Applicable to other firmicutes. journals.asm.org/doi/10.1128/...

The NIH has been holding back grants from funds Congress has already approved.

SUMMARYGlutamate is an essential building block and the most important amino group donor in the cell. The reactions involved in synthesis and degradation link carbon to nitrogen metabolism. The synthesis and activity of the enzymes catalyzing these reactions must, therefore, be precisely regulated. In the Gram-positive model bacterium Bacillus subtilis, glutamate is exclusively synthesized by the combined action of the glutamine synthetase (GS) and the glutamate synthase (GOGAT). The GS catalyzes the ATP-dependent assimilation of ammonium, resulting in the formation of glutamine. Glutamine is converted together with 2-oxoglutarate by the GOGAT into glutamate, which can be used either for further assimilation of ammonium, or as a building block, or amino group donor. The glutamate dehydrogenases (GDHs) GudB and RocG are strictly devoted to glutamate degradation. In recent years, exciting new observations have been made in nitrogen metabolism in B. subtilis. The GS, GOGAT, and the GDHs are multifunctional enzymes, with the GS and GDHs acting as trigger enzymes in the control of gene expression, in addition to their enzymatic activity. The glutamate-synthesizing GOGAT acts as a counter enzyme, inactivating the major GDH GudB to prevent a futile cycle. In this review, we intend to summarize the current state of knowledge about nitrogen metabolism in B. subtilis and discuss open questions that need to be answered in the future.

We are hiring! Focus is on colleagues interested in protein homeostasis and human disease. Priority reviewing starts mid-June and will continue on a rolling basis. Here is the ad, hit me up if you have questions! careers.umass.edu/jobs/assista...

🚨Faculty job search: University of Illinois Chicago Micro & Immuno department is hiring! Open rank and open area within host-pathogen interactions. Apply by May 31 to join us in UI College of Medicine in the heart of Chicago! uic.csod.com/ux/ats/caree...

We are hiring @umichmicroimmuno.bsky.social ! Tenure-track assistant professor position in our Microbiology and Immunology department. We are especially looking for someone working in bacterial pathogenesis