New work from the Moazed lab reveals the requirements for establishment & epigenetic stability of mammalian heterochromatin! www.cell.com/molecular-ce...
Please join us on November 20th! We are very grateful for our first sponsorship - Molecular Instrument!
🧬 Transcription elongation by RNA polymerase II relies on a web of elongation factors. Our new work shows how IWS1 acts as a modular scaffold to stabilize & stimulate elongation. Fantastic work by Della Syau! www.biorxiv.org/content/10.1...
🧬📄 Preprint update (v2): We added new biochemical experiments that clarify how IWS1 associates with the transcription elongation complex and further define competition between IWS1 and RECQL5. 🧪⚙️
www.biorxiv.org/content/10.1...
Structural model is already available in the PDB (9MLC).
My first research paper as an independent PI is now online. Thank you to the authors for contributions and to the reviewers for thoughtful and constructive comments. Grateful to the editor for the opportunity. onlinelibrary.wiley.com/doi/10.1002/...
Harvard Med Cell Biology
Happy to share part of my postdoctoral work at the @lucas.farnunglab.com lab. Great collaboration with @voslab.org and @andersshansen.bsky.social. “Structural basis for CTCF-mediated chromatin organization” www.biorxiv.org/content/10.6...
What an exciting meeting at this month’s Gene Expression and RNA Series (GEARS) in Boston! Thank you to everyone who joined us for an introduction to the HCR™ platform.
Special shoutout to @xiaoliwu.bsky.social, jonmarkert.bsky.social, and @ara-latifkar.bsky.social for helping coordinate the event!
GEARs
Lucas Farnung
Lucas Farnung
Molecular Instruments, Inc.
🧪🧬New preprint We present cryo-EM structures of reconstituted CTCF–nucleosome complexes, showing CTCF dimerization drives nucleosome oligomerization into defined higher-order assemblies. Disrupting CTCF–CTCF interfaces in mESCs reduces looping and impairs differentiation. tinyurl.com/CTCF-nucleos...
⚡New preprint from the lab⚡: A key early checkpoint in gene expression is promoter proximal pausing of RNA polymerase II. For over 20 years, we as field have not been able to recreate pausing under realistic cellular conditions. @robertovn.bsky.social shows that ELOF1 is the missing piece.
The two that first showed us how FACT engages nucleosomes now have their own lab and show us how the remodeler ATRX engages nucleosomes! Incredible resolution with a lot of biochemistry, congrats @codyz.bsky.social @yangliu9.bsky.social and team!
Tatarakis et al. study how H3K9me3 heterochromatin is formed and inherited in mammalian
cells. Using a synthetic heterochromatin assembly system and genetic screens, they
uncover requirements for init...
www.cell.com
Video
🚨 New preprint: GATO-seq did it again! ELOF1 is the missing promoter-proximal factor that confers RNA Pol II resistance to TFIIF. Including ELOF1, DSIF, and NELF in GATO-seq reactions recapitulates promoter-proximal pausing in vitro at physiological conditions for the 1st time.
tinyurl.com/ELOF1
Roberto Vázquez Núñez
Video
A collaborative work with Yang. @yangliu9.bsky.social
ATRX was considered as an orphan remodeler. We think it is truely unique.
www.biorxiv.org/content/10.6...
