//
sign in
Profile
by @danabra.mov
Profile
by @dansshadow.bsky.social
Profile
by @jimpick.com
AviHandle
by @danabra.mov
AviHandle
by @dansshadow.bsky.social
AviHandle
by @katherine.computer
EventsList
by @katherine.computer
ProfileHeader
by @dansshadow.bsky.social
ProfileHeader
by @danabra.mov
ProfileMedia
by @danabra.mov
ProfilePlays
by @danabra.mov
ProfilePosts
by @danabra.mov
ProfilePosts
by @dansshadow.bsky.social
ProfileReplies
by @danabra.mov
Record
by @atsui.org
Skircle
by @danabra.mov
StreamPlacePlaylist
by @katherine.computer
+ new component
ProfilePosts
Loading...
We've come full circle! I began my postdoctoral career by identifying the peptidyl deformylase gene. Today, we show that half of bacterial species harbor multiple PDF genes (up to 7, for always a single Met-tRNA transformylase), and while the role of these PDFs ... academic.oup.com/mbe/article/...
6mo
New preprint out on bioRxiv! www.biorxiv.org/content/10.6... Can conjugative plasmids be used to control plasmid and pathogen spread? Follow me down the rabbit hole that led to this story 🧵
A short research stay in 2019 (back in the pre-COVID era) at José R. Penadés lab turned into the third chapter of my PhD and today it finally sees the light.
Here, we reveal an alternative route for cassette shuffling: natural transformation. Incoming cassettes from isogenic cells can be excised and integrated into integrons, mimicking cassette shuffling without deleting endogenous cassettes. 3/5
Abstract. Peptide deformylases (PDFs) are enzymes that are essential for bacterial viability and attractive targets for antibiotic development. Yet, despit
academic.oup.com
Unraveling the Prevalence and Multifaceted Roles of Accessory Peptide Deformylases in Bacterial Adaptation and Resistance
Bacteria chromosomes contain Genomic Islands that provide virulence, antibiotic resistance, MGE-defence,... They transfer between cells, but the mechanism of most remains elusive. Here we explore the conjugative capacity of these mysterious Genomic Islands. www.biorxiv.org/content/10.6...
Integrons are genetic “biobanks” that bacteria use to adapt on demand. They store cassettes that provide functions from antibiotic resistance to phage defense. Our lab showed that cassette shuffling in SCIs is limited because toxin–antitoxin cassettes keep cassette excision rate low. 2/5
Many thanks to all co-authors! @manon-b.bsky.social @baptistedarracq.bsky.social @EliaBandini @eloilittner.bsky.social @epcrocha.bsky.social @amazeld.bsky.social @celineloot.bsky.social And don't miss the upcoming paper from @mblokesch.bsky.social lab on the same topic! 5/5
3mo
3mo
Our new paper is out in @narjournal.bsky.social We show that natural transformation enables bacteria to shuffle integron cassettes, boosting their phenotypic diversity. academic.oup.com/nar/article/... 1/5
6mo
Mechanistically, incoming ssDNA is a game changer: it promotes attC site folding, triggers integrase expression via SOS, and allows cassette excision without the need for replicative resolution. This suggests both intra- and inter-bacterial shuffling shape integron recombination dynamics. 4/5
4mo
6mo
6mo
6mo
Didier Mazel
6mo
Kevin Debatisse
Kevin Debatisse
Kevin Debatisse
Kevin Debatisse
Kevin Debatisse
Pedro Dorado-Morales
Pedro Dorado-Morales
Manuel Ares-Arroyo
www.biorxiv.org
npj Biofilms and Microbiomes - Targeted elimination of Staphylococcus aureus mastitis infections with synthetic phage-based CRISPR-Cas delivery systems
www.nature.com
Targeted elimination of Staphylococcus aureus mastitis infections with synthetic phage-based CRISPR-Cas delivery systems - npj Biofilms and Microbiomes