Brilliant #PhD opportunities for anyone interested in #chromatin, #genetics or #epigenetics!
Lots of exciting chromatin biology - and great recaps!
How do G4s function at enhancers? In our #proteomics pulldowns, RNA polymerase II subunits were enriched, together with known G4 binding proteins - but not some common architectural factors (e.g. YY1, CTCF) - we confirmed that some RNAPII subunits specifically bind G4s (Fig. 4A, F). 6/9
Thank you @stefanschoenfelder.bsky.social - far too modest!
For the first part of this journey, don't forget to check out genomebiology.biomedcentral.com/articles/10.... to find out how we developed a technique, ViCAR, for mapping 3D interactions specific to #epigenetic features (such as G4s)... 7/9
....then edited them out to show how the regulation of genes in 3D contact was affected. 8/9
Inserting an unrelated G4 structure (Pu27 from the #Myc promoter - as featured in @isabelesain.bsky.social's brilliant work www.pnas.org/doi/abs/10.1...) recovers original globin expression and enhancer function (Figure 3D) 5/9
Many thanks especially to Colm Doyle (now at @embl.org), who began this work with his beta globin locus study and @seanflynn.bsky.social (now of sites.google.com/view/flynnlab/ at @exeter.ac.uk - great things to come) for an incredible journey, as well as all authors at @cruk-ci.bsky.social! 9/9
Come and talk 3D #genome, #epigenetics, #enhancers and #G4s at #FOG2026 at London ExCeL today and tomorrow! festivalofgenomics.com/london/poste... and some fresh stuff in there too!
Beyond carrying out vital research, our staff are involved in lots of science outreach activities every year.
Recently, we hosted pupils for our annual Careers Lab. This programme is designed to inspire the next generation of STEM scientists & supporters.
More: www.cruk.cam.ac.uk/students/car...
