Can CRISPR edits enable precise tuning of plant gene expression? We think: yes.
In our newest manuscript, we measured the effects of >30,000 CRISPR-like promoter mutations in sorghum protoplasts.
Crop improvement often relies on random changes to cis-regulatory DNA. With CRISPR we can target genes directly, but finding desirable edits is hard. We screen CRISPR-like mutations in PsbS, Raf1, and SBPase to find edits that achieve the same thing as transgenic overexpression.
Mapping cis-regulatory mutations at scale in sorghum enables modulation of gene expression - @savagecatsonly.bsky.social go.nature.com/3O7mDEN
go.nature.com
Variant mapping of sorghum promoters identifies CRISPR-accessible mutations that upregulate gene expression.
New preprint from Dave Savage (Savage Lab) and Yun S. Song: Deep models of protein evolution in time generate realistic evolutionary trajectories and functional proteins. Read here: https://ow.ly/iJk950YCF8q
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