Assistant Professor at the University of Copenhagen | Head of the Tissue Gene Regulation Lab | http://tgrlab.org
Robert Krautz
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Dear gene regulation aficionados in the wider Copenhagen 🇩🇰area: Please feel cordially invited to our 3rd Copenhagen Gene Regulation Seminar 👨🏫 next week on Thursday, the 5th of March. 📆 Hope to see you in Panum for 2 exciting talks! 👇
We demonstrate how you can use this atlas 🗺️ together with scE2G-predicted enhancer-to-gene links 🔗 to unravel the gene regulatory landscape 🏔️ of your favourite enhancer, promoter, gene or variant of interest.
In parallel, PRIME predicts active CREs directly from transcription initiation patterns which allowed to build a large-scale atlas 🗺️ of CRE activities across human cell types.🫀🧠🫁👁️
Absolutely thrilled 💣 to share our preprint 🖨️: "Mapping active cis-regulatory elements from transcription initiation events”.
www.biorxiv.org/content/10.6...
We introduce nucCAGE and PRIME to identify active cis-regulatory elements (CREs) and disease-associated, non-coding variants.
As an optimised transcription initiation assay 🧪, nucCAGE is more sensitive to nuclear and unstable capped RNAs – crucially allowing us to enrich for sought-after enhancer RNAs. As a proof-of-principle we dissected cell type-specific 🧫 enhancer activities at the human MYC locus.
Absolutely none of this would have been possible without the Andersson lab, crucially Hjörleifur Einarsson, Natsuda Navamajiti and of course @randersson.bsky.social. Very proud as well for this to be the first preprint 🖨️ of the Tissue Gene Regulation lab.
