🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
Ready for BBCs #AQuestionofScience here @crick.ac.uk 🔬🧬🥼🧪🦠
🔬At #MFS25, Giulia Zanetti from @TheCrick unveiled the molecular architecture of the early secretory pathway using cryo-ET & super-resolution microscopy resolving the debate on COPII vesicles and mapping ER-to-Golgi transport in human cells.
🔗https://mfs2025.com/
We're looking for an awesome computational #postdoc to join our group @PioneerCampus. You'll explore native cells with #cryoET, get long-term funding (5+ years), and live in Munich! For more info, see our new Cell Architecture Lab website:...
We are looking for a PhD student/Postdoc to develop methods of cryo-ET and/or study the structure and function of membrane proteins. Great team at the MDC in Berlin.
It's a broad call, please apply and share!
jobrxiv.org/job/max-delb...
Yes! we reproducibly get ~150nm thin lamella with very few failures and regularly run sessions for ~48hrs.
This revolutionised our segmentation of coated membranes.
Membrain V1 didn't recognise coated membranes - lots of manual editing was needed. Membrain V2 - beautiful segmentation, chefs kiss!
I attended this course last year and cannot recommend it enough!
We have the Aquilos2 which is fab!
Our only complaint is the iFLM filter cubes are optimised for room temperature but the spectra of a number of fluorophores changes at cryo and so their peak excitation and emission wavelengths are filtered out leading to poor signal at cryo vs RT
Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!
Make my day and check it out!
www.biorxiv.org/content/10.1...
#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬
Lorenz Lamm
Katie Downes
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