An excellent new protocol from Kristina Bayer and Richard Wombacher (@wombacherlab.bsky.social)! Their lipid-mediated dual SLIPT and dual SLIPT(NVOC) system offers powerful sequential control of protein recruitment in live cells. 🔬💊🔦
bio-protocol.org/en/bpdetail?...
Congratulations to the first author Philipp Pöschko, master student Caroline Berrou and all co-authors. Special thanks also to Kaisa Pakari and Jochen Wittbrodt for the wonderful collaboration. I am very grateful for the enthusiasm and commitment of everyone involved!
Based on Tsukiji’s SLIPT @shinyatsukiji.bsky.social technology we developed dual-SLIPT as a lipid mimic for defined, sequential protein dimerization on the inner leaflet of the plasma membrane. (2/5)
Happy to start our new bsky.social channel with a recent publication from our lab: Photoinduced protein proximity using novel CIPs - in vivo application in medaka embryos. Check out at ACS Chemical Biology: pubs.acs.org/doi/full/10....
#CIP#chemicalinducerofproximity #photocontrol#chemicalbiology
Great to see this work published in the ACS Chemical Biology special issue Lipids and Lipidation, edited by Jeremy Baskin @jeremybaskin.bsky.social and Bryan Dickinson @chembiobryan.bsky.social. (5/5)
The project was mainly fueled by the passion and enthusiasm of first author Kristina Bayer. In her work Kristina demonstrates the iterative optimization of dual SLIPT for efficiently recruiting proteins for dimerization on the inner leaflet of the PM in a light controlled, sequential manner. (3/5)
With the photo-controllable version of dual SLIPT (dual SLIPTNVOC) we successfully prove the usefulness of the lipidomimetic for the manipulation of cellular processes in the spatiotemporal control of lamellipodia formation. (4/5)
Happy to share our new publication in ACS Chemical Biology @pubs.acs.org reporting a new chemical biology tool – dual SLIPT! Congratulations to first author Kristina Bayer @XXX, all co-authors and collaborator Shige Yoshimura @XXX. Open Access:
pubs.acs.org/doi/10.1021/... (1/5)
Shinya Tsukiji
📣 The application deadline for #EMBLFluorescenceLifetime has been extended until 28 Apr! ✍🏼
Are you a PhD student or a postdoc with experience in fluorescence microscopy and want to enhance your skills in confocal fluorescence microscopy and lifetime-based readouts? 🔬
➡️s.embl.org/lef25-01-bl
Cellular phenomena such as signal integration and transmission are based on the correct spatiotemporal organization of biomolecules within the cell. Therefore, the targeted manipul...
