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CryoARC: Atomic-resolution conformational landscapes of protein assemblies from cryo-EM single particles with evolutionary priors www.biorxiv.org/content/10.64898/2026.05.25.727696v1 #cryoEM
Genuinely is the new delve
Unless you ask PyTorch for determinism (rarely done in production because it's very slow), ops involving scattering won't be deterministic. Do you have a gif comparing 2 outputs with identical postprocessing? The high-pass filter in your example makes it hard to compare denoising specifically.
You've done an amazing job! I really had a blast working with you, Alister, and Miguel.
MissAlignment is out!!! Our new software that improves reference-free tilt series alignment by mimicking human intuition. Check out Dimitry's beautiful thread for a quick summary! And the preprint for more details: www.biorxiv.org/content/10.6... The program: github.com/warpem/miss-...
Patches, projections, L2 maximization: I've done them all. The birds and bananas wouldn't go away. Then @martenchaillet.bsky.social came along, right after finishing his PhD w/ @fridof.bsky.social. I don't want to jinx it, but I think I'm finally done with bananas. The future belongs to pineapples!
This project had an interesting personal angle: over 10 years ago, I joined @fridof.bsky.social's lab as an undergrad to work on reference-free tilt series alignment. I failed. I've been giving it another try with a new idea ca. once every year since then.
What if you're not interested in ribosomes? First, HOW DARE YOU. Second, MissAlignment's got you covered. We took a tiny subset of EMPIAR-11830 containing parts of the nucleus and looked for nucleosomes. Here, further reference-based alignment with M made no difference.