Assistant Prof at University of Zürich
-- host-pathogen interactions across scales --
We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better.
Preprint: www.biorxiv.org/content/10.6...
Code: github.com/warpem/miss-...
🧶 Thread:
Gregor Weiss
Video
Dimitry Tegunov
If you wanna try our new Autogrid High-Pressure Freezing Planchettes ❄️
- you can use the provided drawings & CAD files to produce them in-house 🛠️
- you can now also contact www.wohlwend-hpf.ch to order yours with a shiny gold-coating ✨
The preprint for instructions ➡️
www.biorxiv.org/content/10.6...
Gregor Weiss
I am happy to share a review I recently wrote on the design of peptide binders. It gives an overview of experimentally validated tools and discusses the challenges of why peptide design is more difficult than the design of classical protein binders.
www.chimia.ch/chimia/artic...
We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇
📜The final version of our paper on eCIS firing is out:
www.nature.com/articles/s41...
Congrats to @xujwet.bsky.social @chipericson.bsky.social
#cryoEM #CIS
Really important and relevant study in TB from @danbarberphd.bsky.social and colleagues. We need to re-think many assumptions. Nitric oxide production in mice has been a confounding factor. #tuberculosis
🛠️💉It's time for engineering contractile injection systems! @chipericson.bsky.social & @davidschaumann.bsky.social introduce programmable CIS - PROCIS, combining tail length control, non-native cargo loading, and cell retargeting all in one system! www.biorxiv.org/content/10.6... 🧵 1/5
The really striking part: all three Dip proteins oscillate pole-to-pole, and DipB and DipC also build ring-like structures at midcell. This is reminiscent of the bacterial Min system, which uses oscillation to keep FtsZ centered — but Dip is its own thing (more on that below).
Video
Bacterial contractile injection systems (CIS) mediates cell-cell contacts, but the firing mechanism is unclear. Here, the authors employ a multimodal cryoEM approach and structure guided engineering t...
Some phages are named after gods or rivers, this one was named by journalist Thilo Mischke @thilomischke.bsky.social after his grandma ("Oma Karin") while filming in our lab.
It's a cool phage that should be temperate but became virulent due to a transposon at its lysogeny control! ⛓️💥💪🏼
Max Gutierrez
In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn
🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social&@chipericson.bsky.social trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬
www.biorxiv.org/content/10.1...
Sonja-Verena Albers
Pilhofer Lab 🦠❄️🔬
Alexander Harms
So we blocked IFNγ in Mtb infected macaques and….the animals were fine. Turns out mice overestimate the importance of IFNγ in TB because IFNγ drives iNOS in mice but not macaques or humans. This means we don’t actually know how T cells suppress Mtb. rdcu.be/ff9GB #tuberculosis #immunology
Biohub
Nature Communications - Here, the authors show that IFNγ blockade does not impair resistance to Mycobacterium tuberculosis infection in macaques, opposed to what has been observed in mice, due...
