Investigating mechanisms of eukaryotic cellular quality control with a focus on autophagy @MPIbp via #cryoET, #teamtomo, #cellbiology and #massspectrometry
Wilfling Lab
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In this study we investigate the architecture of aminopeptidase-1 condensates directly inside cells at molecular resolution! We show how transient local structures can mediate phase separation, directly linking amino acid sequence to condensate material properties and biological function.
We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
Huge thanks to all amazing co-authors and collaborators — this work was a true team effort! @mmedina300kv.bsky.social @nanigrotjahn.bsky.social @andschwarz.bsky.social @erin-schuman.bsky.social @sauerkrausi.bsky.social @harperlabhms.bsky.social @sonjawelsch.bsky.social @hummerlab.bsky.social
Also check out a fantastic complementary study from the Jeffery Kelly lab! They independently uncover how LLOMe disrupts lysosomes by forming amyloids that may act as seeds for pathological aggregates
🔗 doi.org/10.64898/202...
Many thanks to first authors @emboyle.bsky.social, @sergiocruzleon.bsky.social, @structjavi.bsky.social and Johann Brenner and our fantastic collaborators from the Kraft @kraftlabfr.bsky.social, Hummer @hummerlab.bsky.social, Milles @millessigrid.bsky.social and Hunte labs for all their great work!
LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery.
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.
🔗 doi.org/10.64898/202...
#CryoET
Excited to welcome the autophagy community in Oslo for the International Autophagy Conference 2026.
📍 Holmen Fjordhotell, Oslo
📅 25–28 May 2026
Looking forward to outstanding science and discussions on the future of autophagy research.
Thanks to our sponsors:
@tandfonline @embojournal @NoAge100
Video
Updated preprint 🧵
We mapped how loss of 23 LSD genes reshapes neuronal proteomes in cortical (iN) and dopaminergic (iDA) neurons
New in v2: We added a computational PPI pipeline assessing KO-dependent vulnerability at the interaction and complex level.
Huge thanks to all the fantastic co-authors and collaborators, especially @harvardcellbio.bsky.social, @harvardcellbio.bsky.social , @wilflinglab.bsky.social, Memorial Sloan Kettering Cancer Center, @citehms.bsky.social @jencwaters.bsky.social, and @hms-iac.bsky.social, @fedegasparoli.bsky.social!
Wilfling Lab
Wilfling Lab
Wilfling Lab
Wilfling Lab
Wilfling Lab
Wilfling Lab
Felix Kraus
ARCTiC: Autophagy Research Centre Targeting Cancer
