Nature research paper: Single-cell and isoform-specific translational profiling of the mouse brain
go.nature.com/4aYPQu7
Still a few days to apply :)
Really exciting times at HT!
Thrilled to announce that Iāve just opened my research lab at @helmholtz-hiri.bsky.social !
A huge thank you to my mentor Roi Avraham (and lab members!) for the incredible training and support that made this possible.
šØIn our new preprint we show that miRNAs act within a precise temporal window to control cell fate decisions in the human forebrain. š§
A study of miRNA expression dynamics and functions during early human brain development. @mdc-bimsb.bsky.social ā¬
Check it out! www.biorxiv.org/content/10.6...
We're looking for a group leader in functional genomics at @humantechnopole.bsky.social
Super exciting science, cutting edge facilities, very competitive package. More info here:
careers.humantechnopole.it/job/Senior-R...
Happy š„²
We're now looking for a postdoc and a technician to work on this super exciting 5 years project on mRNA mtabolism and poly(A) tail length control.
Links to apply here:
Postdoc: careers.humantechnopole.it/job/Postdoc-...
Technician: careers.humantechnopole.it/job/Technici...
Post-transcriptional regulation of mRNA translation was explored using Ribo-STAMP and single-cellĀ RNA sequencing to reveal cell-type-specific and isoform-specific translation patterns across hippocampal neuronal and non-neuronal cell types, highlighting functional differences between CA1 and CA3.
MicroRNAs (miRNAs) are key post-transcriptional regulators of cell state transitions, yet their function in early human brain development is largely unknown. Here, we present a longitudinal analysis of miRNA function in developing human forebrain organoids. We show that mRNAs and miRNAs expression mirrors known developmental gene programs and that miRNA biogenesis peaks at neural commitment. To test the function of miRNAs in regulating commitment, we impaired their biogenesis at defined stages. miRNA disruption during pre-neuronal commitment caused severe patterning defects, whereas post-commitment perturbation had minimal impact on forebrain identity. We show that miRNA loss during pre-commitment increased WNT and BMP signaling, thus shifting cell fates towards non-forebrain identity such as midbrain/hindbrain. These effects could be partially rescued by expressing five miRNAs. Our findings uncover a critical time window where miRNAs regulate morphogen signaling in early human neurodevelopment, establishing them as essential temporal determinants of cell fate and brain regional identity. ### Competing Interest Statement The authors have declared no competing interest.
www.biorxiv.org
Senior Research Group Leader in the Genomics Research Centre - Functional Genomics Programme
Had a great day at Human Technopole - lots of stimulating discussions with students and faculty and also, this should be a new standard, Aperol spritz after the seminar! @humantechnopole.bsky.social
1/6 We're hiring! Fully funded postdoc position in my group
humantechnopole.it/en/research-...
We've spent the last year building something at @humantechnopole.bsky.social that we think is genuinely needed: #AI that works across biological scales and data modalities, not just within them. A thread on what we're doing, why, and who we're looking for. A š§µ... 1/9
humantechnopole.it
Laboratory for Molecular and Systems Biology of RNA Ā The Legnini Group at Human Technopole combines molecular and systems biology approaches to study gene regulation. We use synthetic biology and opt...
š§¬ What controls how long an #mRNA molecule survives inside a cell?
š¬ Our @ilegnini.bsky.social Group leads POLARIS, a new ā¬1.35M FIS3 project exploring how poly(A) tails regulate mRNA stability and gene expression.
Read more š humantechnopole.it/en/news/pola...
Human Technopole has been awarded a ā¬1.35 million Consolidator Grant from the Fondo Italiano per la Scienza (FIS3) to investigate how cells control the stability of messenger RNAs. The funding will su...
