It‘s not always the best solution to do your CRISPR(i) screen at a low MOI (or the dogmatic 0.3-0.5 range 😉) - superinfection can give you a higher quality output!
Super cool to finally see some of @chnebster.bsky.social postdoc work out and published!
Irene Beusch
A project from my postdoc in the McManus lab @mcmanuslab.bsky.social at UCSF @ucsfdc.bsky.social is out today 🎉
For years, pooled CRISPR screens have operated under a central assumption: one perturbation per cell.
We challenged that assumption. 🤯
www.nature.com/articles/s41592-026-03095-w
This paper shows that high MOI sgRNA multiplexing maintains CRISPRi screen performance while enabling reduced cell numbers, challenging conventional reliance on low-MOI infections.
