We have published a review on how contextual information can complement and enhance research using #cryoET .
www.sciencedirect.com/science/arti...
Ready to TANGO? 💃 In our new publication, Beata Turoňová & Markus Schreiber (@ms314.bsky.social, @becklab.bsky.social) present TANGO: the first cryoET framework to analyze the relation between particle positions and orientations 🪩 All in a single user-friendly package! #CryoET #OpenScience
Cryo-electron tomography (cryo-ET) enables the analysis of biological samples in situ, revealing the complex interplay that shapes (sub)cellular archi…
www.sciencedirect.com
Markus Schreiber
Max Planck Institute of Biophysics
#teamtomo strikes again! Check out our new preprint on the enigmatic vault and its newly discovered association with membranes in situ.
Exciting new paper from my colleague @deglushk.bsky.social in the @becklab.bsky.social! It introduces a fast, robust tool for measuring membrane thickness in tomograms #TeamTomo.
PhD: defended ✅🎉 My work used in situ cryo-ET #teamtomo to investigate cellular machines from ribosome translation and HIV capsid at the nuclear pore to membrane association of vault, and the molecular architecture of heterochromatin. Huge thanks to @becklab.bsky.social. What a wonderful ride!
Jan Philipp Kreysing
Jan Philipp Kreysing
Jan Philipp Kreysing
We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
New paper out!
How can we force HIV genomes out of their capsid shells and what can we learn about HIV biology doing so?
Hint: capsid inhibitors actively re-route nuclear HIV complexes, and expose genomes via intriguing structural effects!
open access:
link.springer.com/article/10.1...
read below
Out now in Nature Communications!
We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky
www.nature.com/articles/s41...
Updated architectural model of the NPC from our lab, starred by bsky-less Agnieszka Obarska-Kosińska. Using cryo ET, XL-MS and modeling, we identify novel nucleoporins, among those the mRNA export complex TREX-2! Check out the preprint on biorxiv #TeamMassSpec #TeamTomo
We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better.
Preprint: www.biorxiv.org/content/10.6...
Code: github.com/warpem/miss-...
🧶 Thread:
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Wilfling Lab
Thorsten Müller
Katharina Geißler
Katharina Geißler
Dimitry Tegunov
Following cell entry, HIV-1 capsids enter the nucleus by passage through nuclear pores and reach nuclear speckles with subsequent uncoating of the reverse-transcribed genome and its integration into s...
It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...
Katharina Geißler
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
Video
Desislava Glushkova
How the TREX-2 complex associates with the nuclear pore https://www.biorxiv.org/content/10.64898/2026.02.20.707084v1
Video
bioRxivpreprint
The vault associates with membranes in situ https://www.biorxiv.org/content/10.64898/2025.12.12.693869v1
