See also NRF2 and Nrf2 (Nuclear Respiratory Factor vs. Nuclear factor erythroid 2-related factor 2). Totally different proteins that get mixed up all the time!
pubmed.ncbi.nlm.nih.gov/38815934/
A group of research integrity activists say they have found widespread evidence of image manipulation in Thermo Fisher Scientific’s catalog of antibody reagents.
#chemsky 🧪
The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes:
🖌️ Dozens more images with duplications or painting
🖨️ Hundreds of blots that all share the same background (behold slideshow below)
Weekend reads: White House proposal prohibits federal funds for APCs; sleuths say Thermo Fisher doctored data; sleuth in China takes to social media
New @biorxivpreprint.bsky.social preprint led by @pranujanp.bsky.social, studying errors such as incorrect primer sequences and contaminated #CellLines in #circRNA papers in high impact factor journals. We found reagent errors in 51% of n=118 circRNA papers in high IF journals in 2022 (1/4)🧪
In recent years, the acronym NRF2 has garnered significant attention in scientific discourse. However, this attention has occasionally led to confusion due to the existence of two distinct proteins sh...
pubmed.ncbi.nlm.nih.gov
Apparent manipulation of validation images in reagent catalog concerns researchers
@marshall0i.bsky.social
I believe this is another story that you can use for your course.
100s labs ordered the wrong "p16" antibody.
They wanted to study p16-INK4a, but bought p16-ARC antibodies.
312 papers got it wrong.
Now the literature is polluted with false mechanistic pathways.
As a reminder: validate your reagents.
But also read the datasheet and use your brain.