SeeDB-Live is a tissue-clearing approach for live tissue slices or brains, improving image quality while having minimal effects on electrophysiological properties of neurons. @imailab.bsky.social
www.nature.com/articles/s41...
SeeDB-Live is a tissue-clearing approach for live samples such as tissue slices or the in vivo brain. It improves image quality while having minimal effects on electrophysiological properties of neuro...
A Clear Solution: Seeing living brain cells fire signals at high magnification by matching the refractive index between tissue & lens
📹 Shigenori Inagaki et al @imailab.bsky.social, Kyushu University in @natmethods.nature.com
➡️ bpod.org.uk/archive/2026... with
@rooph.bsky.social
Video
Biomedical Picture of the Day BPoD
SeeDB-Live enabled us to perform 1P functional imaging of brain slices.
This movie shows 1P voltage imaging (Voltron2) of back-propagating action potentials in mitral cell dendrites, captured with a high-speed CMOS camera at 2 kHz. Single shot, no averaging. (7/10)
A step-by-step protocol has been posted in SeeDB Resources:
sites.google.com/site/seedbre...
The reagent will soon be commercially available from Nacalai Tesque.
www.nacalai.com/global/index...
We hope that our new tool will spark new applications in bioimaging!
(10/10)
We are grateful for the invaluable help from our collaborators.
More stories behind our findings:
Research Briefing: www.nature.com/articles/s41...
EurekAlert!: www.eurekalert.org/news-release...
The Transmitter: doi.org/10.53053/EIM...
(9/10)
We also successfully cleared the cerebral cortex of live mice. The brightness was 3x higher in L5. Sensory responses remained unchanged. We also established chronic imaging using a repeated clearing protocol, without any obvious inflammation or behavioral abnormalities. (8/10)
SeeDB-Live is useful for functional imaging of brain slices. This movie shows 2P imaging of GCaMP6f in mitral cells during the clearing of an olfactory bulb slice. Spontaneous activity was more clearly visualized after clearing, while the firing pattern remained unchanged. (6/10)
Interested in live tissue clearing?
SeeDB-Live is now commercially available from Nacalai Tesque!
Product page: www.nacalai.co.jp/products/445/
Protocol: sites.google.com/site/seedbre...
Video
Video
Making live mammalian tissues transparent for imaging experiments without compromising their normal cellular functions has been a long-standing challenge. An isotonic and minimally invasive optical cl...
Publications
Inagaki et al., Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo [bioRxiv preprint]
Raw image data: https://ssbd.riken.jp/repository/484/
Using patch-clamp recording, we confirmed that neuronal firing properties remain largely unchanged after clearing. This was a tough experiment, because neurons become invisible after clearing. Nao Nakagawa-Tamagawa worked very hard to patch invisible neurons in cortical slices! (5/10)
Takeshi Imai
Takeshi Imai
Takeshi Imai
Takeshi Imai
To minimize osmolarity, we screened high-MW chemicals and found that BSA has an exceptionally low osmolarity. After fine-tuning the ionic compositions, we established a live tissue clearing agent, SeeDB-Live. The movies show the clearing of a HeLa cell spheroid. (4/10)
Our live tissue clearing paper is out in @natmethods.nature.com! We achieved optical clearing of mammalian brain tissues without compromising normal neuronal function. Big congrats to Shigenori Inagaki and our wonderful collaborators! 🎉
www.nature.com/articles/s41... (1/10)
