4/ We propose that this conserved architecture reveals a strategy for metabolic compartmentalization that couples efficient catalysis with increased molecular robustness.
3/ Structure-guided perturbation abolishes the assembly in vivo without measurably altering intrinsic catalytic activity, suggesting that the main role of the assembly is spatial organization rather than allosteric regulation.
2/ Urate oxidase homotetramers assemble into helical fibers that laterally tile into a stable, porous lamellar scaffold. This organization preserves accessible surface area while allowing substrate and product exchange.
Preprint with new server~ Using in situ cryo-ET, we identify a previously uncharacterized higher-order architecture of urate oxidase in mouse liver.
langtaosha.org.cn/lts/en/prepr...
Cells organize metabolism within a crowded intracellular milieu, yet the structural logic and functional consequences of supramolecular enzyme assemblies in vivo remain poorly defined. Here, using in
