I'm excited that this collaborative work with Alan @acartmell47.bsky.social is finally out. We have developed some powerful tools to study carbohydrate sulfatases (a tricky class of enzyme!) and shown how they can be used with enzymes from host-associated bacteria.
Zach Armstrong
Happy to have this collaborative work with Zach Armstrong now out in Angewandte chemie doi.org/10.1002/anie... .
We use a linked assay, coupled with fluorescent probes, to analyse mucin active carbohydrate sulfatase kinetics, cellular location, and inhibition.
We report plate-based coupled assays to rapidly and quantitatively determine the activity and selectivity of mucin-active carbohydrate sulfatases. This fluorogenic assay further enables inhibitor ide...
