How do you know if a microscopy experiment can actually resolve a biological structure?
Introducing VLab4Mic, a platform that predicts structural resolvability before you go to the microscope
Led by Damián Martinez and Bruno Saraiva at @henriqueslab.bsky.social
www.biorxiv.org/content/10.6...
A final note:
This is my first paper to come out as a new PI at @aboakademi.bsky.social, and I couldn't be happier! 🎉☺️😊 #aboakademi
Mario Del Rosario
Workflow:
🧬 Import structure
🏷️ Choose labelling strategy
🎲 Simulate realistic stochastic labelling
🔬 Generate virtual images for Widefield, Confocal, AiryScan, STED and SMLM
Helping researchers test experiments before collecting data vlab4mic.henriqueslab.org
Congratulations to Damián Martinez, Bruno Saraiva, @taylashakespeare.bsky.social, @wmarkbates.bsky.social, Dylan Owen, @christlet.bsky.social and @henriqueslab.bsky.social
We hope VLab4Mic helps researchers design better experiments and ask better biological questions
💻 vlab4mic.henriqueslab.org
Can microscopy distinguish a flat from a domed clathrin lattice?
VLab4Mic predicts:
❌ Confocal
❌ AiryScan
✅ STED
✅ SMLM
A simple example of how simulation can guide modality selection before spending microscope time
Resolution isn't just optics. Structural resolvability also depends on probe size, linkage error, labelling efficiency, steric hindrance and, molecular organisation. VLab4Mic models all of these starting from PDB or AlphaFold structures
Mario Del Rosario
It was a pleasure to help @mariodelr.bsky.social, @henriqueslab.bsky.social and everyone on this new way to think and plan our microscopy experiments!
Mario Del Rosario
Mario Del Rosario
Mario Del Rosario
Mario Del Rosario
🔬🚀 It's out! Run 100s of imaging experiments before touching a microscope. #VLab4Mic reproduces them in silico from PDB/AlphaFold to help you image right first time. From rockstars Damián Martínez & Bruno Saraiva, inaugural preprint of @mariodelr.bsky.social new lab
www.biorxiv.org/content/10.6...
