I created a Metabolomics starter pack. A list of researchers from the wonderful world of #metabolomics. If you would like to be added (or removed) just let me know. go.bsky.app/J3VPYKm
2/ We adapted Low-Temperature Thermal Evaporation (LTE)—originally used in nanotechnology and solar cell applications—for matrix deposition in #MALDI-MSI. The result: a reproducible, vacuum-based method that offers precise control over matrix thickness and produces ultra-pure coatings.
1/ In MALDI-MSI, matrix deposition is everything. It impacts sensitivity, spatial resolution, and reproducibility. We asked: can we improve matrix application using a dry, solvent-free, controlled method?
3/ We validated LTE using two matrices:
✅ DHB
✅ DAN
Calibrated thickness vs. deposition time = ✔️ reproducibility.
4/ ESEM images showed beautiful, uniform sub-micron matrix crystals across the tissue. Small crystals = better ionization = sharper images.
New paper out in Briefings in Bioinformatics
📰SingleFrag: a deep learning tool for MS/MS fragment and spectral prediction and metabolite annotation academic.oup.com/bib/article/...
We’re excited to share our latest work in @jasms.bsky.social:
“Improving MALDI Mass Spectrometry Imaging Performance: Low-Temperature Thermal Evaporation for Controlled Matrix Deposition and Improved Image Quality”
🧵👇 pubs.acs.org/doi/10.1021/...
5/ LTE purifies the matrix during deposition — great for improving signal-to-noise, even when starting with lower-purity matrix
6/ The matrix stays stable at −80°C for at least 2 weeks. No loss in ionization efficiency or image quality after storage — big win for throughput & experimental planning