Scientist, mum, wife. Fan of gut bacteria, mycobacteria, glycans and cazymes. Mannose evangelist. Lowe Lab, NUBI, Newcastle University
Lis Lowe
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I'm excited that this collaborative work with Alan @acartmell47.bsky.social is finally out. We have developed some powerful tools to study carbohydrate sulfatases (a tricky class of enzyme!) and shown how they can be used with enzymes from host-associated bacteria.
Happy to have this collaborative work with Zach Armstrong now out in Angewandte chemie doi.org/10.1002/anie... .
We use a linked assay, coupled with fluorescent probes, to analyse mucin active carbohydrate sulfatase kinetics, cellular location, and inhibition.
We report plate-based coupled assays to rapidly and quantitatively determine the activity and selectivity of mucin-active carbohydrate sulfatases. This fluorogenic assay further enables inhibitor ide...
Time for @kateduncan.bsky.social to talk about chemistry and why microbes use it via some interactive questions
Zach Armstrong
Alan Cartmell
Funny how absence can tell its own story. Seeing the university display the Pride flag right opposite my window was heartwarming every June. It's been two years now without it. Sign of the times I guess.. 🏳️🌈
Happy to have this collaborative work with Zach Armstrong now out in Angewandte chemie doi.org/10.1002/anie... .
We use a linked assay, coupled with fluorescent probes, to analyse mucin active carbohydrate sulfatase kinetics, cellular location, and inhibition.
Doing the periodic cleaning of used plasmid miniprep columns and realized in honor of Earth Day it may be worth reminding you molecular biologists:
Plasmid miniprep columns can be cleaned and reused essentially indefinitely! Just add 1 M HCl, soak overnight and wash 3x w/ water. Save $ and plastic
Our study on Bacteroides iron piracy is now out in @pnas.org. We show how surface-exposed lipoproteins bind xenosiderophores with different iron-chelating chemistries and propose a mechanism for xenosiderophore import across the outer membrane. Lots of structures (mostly X-ray this time)!
Don't be shy to take on a little two-week side project. These five months will be the most precious three years of your academic journey.
