Current: E11 Bio. Previously: PhD MIT with Ed Boyden and Fei Chen. I develop technologies to accelerate biological research.
Andrew Payne
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In our first study, we acquired a unique dataset in mouse hippocampus. Barcodes improved the accuracy of tracing genetically labelled neurons by 8x – with a clear path to 100x or more. They also permit tracing across spatial gaps – essential for mitigating tissue section loss in whole-brain scaling.
We discovered a new cell barcoding approach exceeding comparable methods by more than 750x. This is the heart of PRISM. We integrated this capability with microscopy and AI image analysis to automatically trace neurons at high resolution and annotate them with molecular features.
Preprint: www.biorxiv.org/content/10.1...
Portal and gallery: www.e11.bio/tech
Addgene constructs: www.addgene.org/depositor-co...
Volara: github.com/e11bio/volara
Open data: github.com/e11bio/e11-o...
Thanks to AWS for hosting our data sets as described in their blog post:
aws.amazon.com/blogs/public...
Congrats to Arlo Sheridan and Will Patton for releasing Volara, our first open-source library for scalable image processing, and also for a great technical writeup!
E11 Bio is excited to unveil PRISM technology for mapping brain wiring with simple light microscopes. Today, brain mapping in humans and other mammals is bottlenecked by accurate neuron tracing. PRISM uses molecular ID codes and AI to help neurons trace themselves.
Read more: e11.bio/blog/prism
Andrew Payne
Andrew Payne
Video
Wonderful to collaborate with, and to support as an advisor, E11 Bio - and to announce PRISM, a technology for mapping brains in a self-correcting way, by barcoding neurons followed by expansion microscopy! Thread below by E11 Bio CEO Andrew Payne, with preprint at, www.biorxiv.org/content/10.1...
