Inlay

Tenured Scientist in Synthetic Biology (INRAE, Paris Area, France) #CellFree ⦁ #SynBio ⦁ #Bioengineering ⦁ #Microbiology https://sites.google.com/view/olivierborkowski

📢 Institut Jacques Monod Seminar 📅 June 12th 📍 Institut Jacques Monod Invited by the @romet-jegou-lab.bsky.social Lab, @lblanchoin.bsky.social and @manuelthery.bsky.social will present the IJM seminar "The self-renewing cell: how actin networks are built, sized, and dismantled " ➡️ buff.ly/T8li591

Genome-guided generative adversarial learning enables nanopore adaptive sequencing doi.org/10.1038/s414...

Reconstructing heterogeneous metabolic trajectories of E. coli diauxie via a dynamical Maximum Entropy Principle doi.org/10.64898/202...

Bioluminescent sentinel plants enable autonomous diagnostics of viral infections doi.org/10.1038/s414...

Generative design of synthetic gene circuits for functional and evolutionary properties doi.org/10.1038/s415...

A natural depsipeptide antibiotic binds the E-site of the bacterial ribosome doi.org/10.1038/s415...

International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity doi.org/10.1126/scia...

Characterization of a Two-Sided Orthogonal Translation System and Its Application to Violacein Biosynthesis pubs.acs.org/doi/10.1021/...

Pao-Wan and co's paper is published now as well ! "A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications" www.nature.com/articles/s41...

In a new study, researchers describe a bacterial enzyme that synthesizes DNA without a nucleic acid template, using its own structure as a guide. Learn more on #DNADay: https://scim.ag/3OEc2RV

Institut Jacques Monod

Improved fractionation strategies can identify antibiotics with previously unseen scaffolds and mechanisms, exemplified by manikomycin from Streptomyces rimosus, which acts by targeting the E-site of ...

npj Systems Biology and Applications - Generative design of synthetic gene circuits for functional and evolutionary properties

A major challenge in the microbial production of valuable compounds is the trade-off between cell growth and biosynthesis. Orthogonal translation systems, which utilize orthogonal ribosomes derived from engineered rRNAs to translate specific mRNAs, offer a means to reallocate translational resources from cellular growth to biosynthesis. However, the in vivo orthogonality and quantitative effectiveness of orthogonal translation systems have not been fully evaluated. Here, we systematically tested orthogonal rRNA–mRNA pairs in Escherichia coli using sfGFP as a reporter and identified a pair that exhibits robust two-sided orthogonality and high translation efficiency. We then applied this orthogonal translation system to the violacein biosynthetic pathway. The orthogonal translation system increased violacein production by 6.30- to 7.77-fold compared with the endogenous translation system and sustained production after entry into the stationary phase. These results demonstrate that a well-characterized two-sided orthogonal translation system successfully improved the productivity of a multienzyme pathway by reallocating translational capacity in vivo.

Multisite deployment of distributed cell-free biomanufacturing expands access and equity in global health research.

T7 RNA polymerase is widely used in synthetic biology, but tools to precisely regulate it are limited. Here, authors engineer a modular T7 RNAP regulatory toolbox for programmable repression, activati...