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by @danabra.mov
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by @jimpick.com
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by @atsui.org
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And here it is: the final protocols for your future favorite 16S rRNA gene primer pair for anplicon sequencing studies -> V4EXT. P.S. Mighty are the small ones - metric💩tons of Patescibacteria everywhere. Except in 💩, that is. academic.oup.com/ismecommun/a...
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Abstract. Primer bias in 16S rRNA gene amplicon sequencing can distort microbial diversity estimates by underrepresenting key taxa. We introduce a modified
academic.oup.com
Revised 16S rRNA V4 hypervariable region targeting primers enhance detection of Patescibacteria and other lineages across diverse environments
Petra Pjevac