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Forgot spike-ins in your lrRNA-seq experiment? 😅 No problem! Our TUSCO benchmarks your transcript models using endogenous single-isoform genes as internal ground truth. Integrated in SQANTI3 for human & mouse. 🧬 Just out in @natcomms.nature.com @pacbio.bsky.social @nanoporetech.com rdcu.be/fkZCb
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Nature Communications - Long-read sequencing enables comprehensive transcriptome characterization but remains challenging to benchmark due to sequencing errors, sample variability, and the limited...
TUSCO: benchmarking transcriptome reconstruction with endogenous single-isoform controls
Ana Conesa