Today, it was Lisa's turn to defend her PhD thesis and she did an absolutely fantastic job 😍 Especially going into full structure of the RNA Pol2 catalytic core and explaining the mutants. It was an outstanding discussion! I am really proud of her performance today 🥹
Excited to share our latest work in Molecular Cell: www.cell.com/molecular-ce...
We show that PRC1 condensates exclude SWI/SNF from chromatin, revealing a physical mechanism for how opposing regulators compete to control gene expression.
Huge thanks to all collaborators and mentors!
Johannes Stein's new lab at the MPIMG will use microscopy to study the organization of the genome at a molecular level. We talked to him about how, despite being a trained physicist, he ended up in biology, the potential of his research and the power of interdisciplinary teams. @jmstein.bsky.social
The next iteration of O-MAP targeting genomic loci is now at eLife!!
DNA O-MAP enables proximity labeling at specific DNA loci took a ton of work from many lab members and collaborators, so it's really exciting to wrap up this paper with @oligopain.bsky.social!
elifesciences.org/articles/102...
Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort.
www.biorxiv.org/cgi/content/...
Very excited to share our newest findings! A very long team effort pursued by multiple team members -to frantically reproduce everything over and over-, we show mTOR-Polycomb synergy at promoters of developmental master regulator TFs in ESCs prior to their expression. www.biorxiv.org/content/10.6...
Out now in Science! Our study challenges long-standing assumptions about transcription factor specificity in eukaryotes. Novel single-molecule measurements of TF behavior in living cells reveal an independence of locus-specific binding from DNA sequence recognition.🧵
www.science.org/doi/10.1126/...
Please check out our newest work on fluorogenic speed imager probes, in which we combine the strengths of speed-optimized imagers and fluorogenic imagers.
#SuperResolution #DNAPAINT #microscopy
www.biorxiv.org/content/10.6...
Our most recent work on the “function and evolution” of #nuclear-speckles is now online at Cell @cp-cell.bsky.social
doi.org/10.1016/j.ce...
Read the thread👇 for the highlights of our findings.
It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density. Lipid-CLEM, now out in @natcellbio.nature.com brought to you by @mathilda95.bsky.social changes that:
www.nature.com/articles/s41...
Niekamp et al. show that biomolecular condensates formed by Polycomb repressive complexes
can physically restrict access of the activating remodeler SWI/SNF to chromatin. Establishing
this barrier dep...
Johannes Stein joined the MPIMG in 2025. His lab will use state-of-the-art microscopy techniques to examine the organisation of the genome at a molecular level. We talked to him about how, despite bei...
How intrinsically disordered regions (IDRs) shape chromatin binding and nuclear organization of transcription factors (TFs) remains unclear. We used proximity-assisted photoactivation (PAPA), a single...
DNA O-MAP enables proximity labeling at specific genomic loci in fixed cells using programmable oligonucleotides, revealing locus-proximal proteomes and chromatin interactions without genetic modifica...
