Interdisciplinary scientist interested in technology development, microscopy and biological sciences.
Multispectral live cell imaging of membrane trafficking.
MRC LMB, Cambridge, UK.
Akaash Kumar
Loading...
Great work from Lucas, the @simonbullock11.bsky.social lab and collaborators uncovering the relocalisation of organelles by dynein when cells experience stress! Check out the pre-print 👇🏽
Myself and @immunoah.bsky.social are looking to hire a postdoctoral scientist to build a next-generation high-throughput high-resolution light sheet microscope for imaging entire cleared organs/tissues and to further develop live & fixed tissue clearing methods.
www.nature.com/naturecareer...
We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:
Congratulations @lara-kruger.bsky.social ! We wish you all the best!
Thank you very much for the feature and the article!! A nice surprise before the weekend...
Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!
We demonstrated the ability to simultaneously image trafficking of five different endogenous receptor classes using de-novo binders from David Baker's lab, by adding the multispectral technology to a state-of-the-art single objective light sheet microscope, capturing data at 3 volumes per second.
🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
The technology we developed is compatible with any camera-based microscope and by using polycistronic plasmids and live-cell dyes (e.g. @spirochrome.com) we can image multiple organelles in live cells simultaneously.
In future work, I am now using our multispectral technology to study membrane trafficking pathways in cytotoxic T cells and related immunotherapies, to decode how T cells are able to eliminate virally-infected/cancer cells and what happens in patients when these processes go wrong. Updates to come!
Akaash Kumar
A tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with...
