Assistant Professor at @isbscience.org studying single-cell lifestyles of bacteria.
Anna Kuchina
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Paper out: Profiling of lytic phage infection in individual B. fragilis cells with bacterial scRNA-seq. Congratulations Anika Gupta, Norma Morella, @monochamussutor.bsky.social Dmitry Sutormin et al, in collaboration with Georg Seelig (UW) and Neel Dey (Fred Hutch). www.nature.com/articles/s41...
Phage-bacteria interactions are typically studied in bulk culture, which obscures cell-cell differences. Here the authors study phage-bacteria interactions using single-cell transcriptomics, identifyi...
We are excited for mapSPLiT to enable genome-scale genetic screens with transcriptomic readouts for bacteria in the future (just like eukaryotes). Amazing work Jacob Brandner, Quoc Tran, Yujia Huang, Dmitry Sutormin and Karl Gaisser from @isbscience.org and UW. 4/4
Anna Kuchina
Rubbing shoulders with a @nobelprize.bsky.social laureate! 🤩What a week!
Couldn’t have happened to a nicer person: isbscience.org/news/press-r...
@isbscience.org @naehasubramanian.bsky.social @flash-point.bsky.social @annabiosys.bsky.social
NYT feature on their website's front page about our local C. elegans colleague Will Mair and NIH funding
www.nytimes.com/2026/03/13/u...
We sat down with Anika & Dmitry to discuss the new paper where we use bacterial scRNA-seq for studying bacterial phage infection and defenses. It turns out that bacteria use pre-existing variation in multiple layers of traits to protect themselves from unpredictable phages @isbscience.org
Dmitry explains how surprisingly universal functional patterns emerge once you look closer into a bacterial community. @isbscience.org
What an astonishing achievement, congratulations Mary! @isbscience.org
mapSPLiT also uncovered the genetic interactions between multiple genes via a combinatorial screen of double and triple perturbations. Finally, we show that mapSPLiT can be ported to a less studied bacterium P. putida, revealing new responses to a perturbation of a carbon metabolism regulator. 3/4
mapSPLiT works by detecting a barcode construct specific to the perturbation alongside the transcripts in each single cell using microSPLiT, a bacterial scRNA-seq method. We used mapSPLiT to map gene expression responses to 118 perturbations in a single experiment in E. coli. 2/4
