The case study highlights several approaches that can help uncover hidden populations within a #cryo-EM dataset, summarized in the latest CryoSPARC Blog post: cryosparc.com/blog/case-st...
Structura Biotechnology
A single cryo-EM grid may contain multiple molecular species, distinct conformational states, interaction partners, or oligomeric assemblies. When properly characterized, this heterogeneity can provide far more biological insight than a single consensus reconstruction.
Combined with biochemical and in vivo studies, the work identifies promising antibody candidates with both prophylactic and therapeutic potential, opening new avenues for combating measles after exposure.
🚀 Beyond their clinical implications, these findings demonstrate the power of #cryo-EM to uncover the molecular interactions that drive immune protection and guide the development of next-generation therapeutics.
🔍 This recent study provides new insights into how the human immune system recognizes and neutralizes measles virus. Using #cryoEM, the researchers visualized human antibodies bound to viral surface proteins, revealing the molecular mechanisms underlying antibody responses against infection.
🩺 Despite the availability of effective vaccines, measles remains a major global health concern. Recent outbreaks have highlighted the continued threat posed by this highly contagious virus.
In this latest case study, depending on the processing strategy, the same dataset can reveal active and deactive conformational states of complex I, as well as additional respiratory-chain complexes that co-purified with the target protein.
🦠 How does the human immune system defeat measles? #cryoEM and #CryoSPARC reveal the answer. 🔬 A recent study from @lji.org provides new insights into how the human immune system recognizes and neutralizes measles virus. Read more in the latest #CryoSPARC Blog 👉 cryosparc.com/blog/measles
🔍 Sample heterogeneity is both a challenge and an opportunity in #cryoEM data processing. In our latest #CryoSPARC case study we explore EMPIAR-10927, a dataset with acetogenin-bound mitochondrial Complex I, that reveals many more complexes according to the data processing strategy 👉 bit.ly/4uihh8V
