New Article! Concurrent positional dynamics and activity mapping of DNA-binding proteins
New Article! On-resin assembly of cysteine-reactive linkers for controlled site-selective antibody bioconjugation
New Article! Three-dimensional direct neuronal reprogramming for modeling Alzheimer’s disease neuropathology
New Article! Robust analytical methods for bis(monoacylglycero)phosphate profiling in health and disease
New Article! Structural variant calling using Sniffles2
New Article! Label-free interferometry platform for drug response profiling of bioprinted tumor organoids at single-organoid resolution
We are 20!! A Milestone Birthday for Nature Protocols this month, which you can read about in our Editorial www.nature.com/articles/s41..., as well as hearing all about what our Authors think on our new Testimonials page www.nature.com/nprot/testim...!
New Article! An immunocompetent bone marrow-on-a-chip model for studying human hematological malignancies and preclinical therapeutic screening
“My interest isn't really open science; it's high-quality, rigorous science..often that happens to be open.”
Sense from @casey.greenelab.com
that aligns with how serious scientists see research and the idea it should be "open as possible, closed as necessary" www.springernature.com/gp/researche...
Nature Protocols, Published online: 08 June 2026; doi:10.1038/s41596-026-01362-wSniffles2 is an open-source software for reliable detection of structural variants (50 bp and upward) from long-read sequencing. Sniffles2 is able to call variants over a wide variant allele fraction (5–100%) and across multiple samples (population).
Happy 20th birthday, Nature Protocols! @natprot.nature.com
Reading your editorial and author testimonials this morning is a great reminder of how a few kind words really can make such a difference. What a joy to read!
www.nature.com/articles/s41...
www.nature.com/nprot/testim...
Nature Protocols
Professor Casey Greene shares his perspective on how open science practices enable better, more rigorous science that ultimately leads to “more
Nature Protocols, Published online: 09 June 2026; doi:10.1038/s41596-026-01359-5Concurrent positional dynamics and activity mapping (C-DAM) allows simultaneous tracking of protein location, binding dynamics and enzymatic activity on DNA substrates by integrating optical tweezers with confocal fluorescence microscopy.
Nature Protocols, Published online: 10 June 2026; doi:10.1038/s41596-026-01388-0This protocol presents a method for converting fibroblasts from patients with Alzheimer’s disease (AD) into cortical neurons, enabling the investigation of the molecular events underpinning AD-associated neurodegeneration and therapeutic testing.
dlvr.it
Nature Protocols, Published online: 09 June 2026; doi:10.1038/s41596-026-01376-4Site-selective cysteine modification enables the controlled attachment of functional payloads to antibodies. This Protocol describes the synthesis of tetra-divinylpyrimidine (tetraDVP) cysteine-reactive linkers for single payload incorporation.
Since Nature Protocols launched in 2006, protocol sharing has shifted from the margins to mainstream practice. Before the launch of online protocol journals, researchers relied on protocol textbooks, ...
Nature Protocols, Published online: 10 June 2026; doi:10.1038/s41596-026-01379-1Bis(monoacylglycero)phosphate (BMP) is an anionic phospholipid found in late endosomes and lysosomes that is dysregulated in an array of diseases. This protocol describes LC/MS analysis of BMP using critical biological control samples.
dlvr.it
Nature Protocols, Published online: 11 June 2026; doi:10.1038/s41596-026-01387-1This protocol enables users to build a 3D microfluidic human bone marrow-on-a-chip microphysiological system with biomimetic tissue strutures, which enables in vitro study of human hematological malignancies and preclinical therapeutic screening.
Nature Protocols, Published online: 04 June 2026; doi:10.1038/s41596-026-01375-5This is a protocol to bioprint cells within thin, flat extracellular matrix squares in 96-well plates to establish three-dimensional organoid cultures for efficient, high-throughput, time-resolved quantitative phase imaging at single-organoid resolution.
