Excited that our work on RNAPII clusters during development is finally in print today! Thanks to everyone who worked incredibly hard on this project and the community for their engaging (and fun!) discussion during each iteration. Please see the post below for details!
Apratim (Appy) Mukherjee
@mukherja.bsky.social work on RNAPII clusters is in print today: www.nature.com/articles/s41...
Congrats!
TLDR: RNAPII clusters represent transcriptonally engaged molecules at single genes, not super-stochiometric assemblies.
Please check it out and see the the thread below for a summary.
Live molecular-scale imaging in fly embryos shows that a single RNA Polymerase II cluster stays bound to active genes during transcription. Cluster formation requires initiation, and intensity represe...
(1/3) Excited to post our updated pre-print! We show RNAPII clusters are simply collections of polymerase molecules stably engaged at single genes & cluster intensity is strongly correlated with txn output during Drosophila embryogenesis. www.biorxiv.org/content/10.1...
Apratim (Appy) Mukherjee
In eukaryotic nuclei, transcription is associated with the clustering of RNA Polymerase II (RNAPII) molecules. The mechanisms underlying cluster formation, their interactions with genes, and their imp...
1/n Excited to share our new preprint! Using live imaging in Drosophila embryos, we show that RNA Pol II clusters switch from sites of initiation to elongation during zygotic genome activation and that they are stably associated with an active gene: www.biorxiv.org/content/10.1...
