This is interesting...our experience is we get devitrification under similar thermal annealing cycles (although we used buffered protein solutions instead of whole cells, so one possible difference is the intrinsic cryoprotectant ability of cells)
klbr
Surface ice ruining your cryo-ET lamellae? We showed you can sublimate it away — inside the SEM, no extra hardware, no devitrification. 🧊→✨
New preprint from @KarolinskaInst 3D-EM 🧵👇
