🇳🇿🇳🇿 Postdoctoral fellow in the Dixon lab at the Salk Institute.
Tessa Popay
2: NIPBL regulates distinct sets of lineage-related genes across different cell types, likely by facilitating a unique spatial organization only at these select genes. This includes strong enhancer-promoter contacts and reduced insulation across the TSS, possibly reflecting multi-way contact hubs.
1.2: Loops rapidly lost with NIPBL depletion (transient) are associated with active promoter chromatin states, whereas the persistent loops are associated with H3K27me3-marked (Polycomb) chromatin, which may influence the stability of cohesin at this subset of loops.
1.1: A group of cohesin- & CTCF-dependent chromatin loops persist for >4 hours in the absence of NIPBL, but require
NIPBL for establishment during mitotic exit. The persistence of these loops is increased by STAG2 knockout & decreased by STAG1 knockout. We speculate that these are long-lived loops.
Very excited to share my postdoc research in the @jesserdixon.bsky.social lab at @salkinstitute.bsky.social, out online at @natgenet.nature.com today! www.nature.com/articles/s41... We investigated the function of the cohesin accessory protein NIPBL, making two particularly interesting findings:
Acute depletion of NIPBL reveals a class of chromatin loops that are independent of NIPBL for their maintenance but not their establishment and that NIPBL is necessary for the expression of lineage-de...
