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Check out the plasmids for pIGLET, a reproducible and efficient approach to generate zebrafish transgenic lines using site-specific recombination!
3mo
Addgene
The key plasmids are also available via Addgene: https://www.addgene.org/browse/article/28243795/ https://www.addgene.org/68310/ (original phiC31 integrase template from Bischof et al., 2007) Going forward, the Mosimann Lab will be pointing the majority of inquiries for these lines to ZIRC. (2/6)