It's cool to see we aren't the only ones that think rationally designed probes + gapfilling is the future. Check out a corroborating workflow from @mgrillo.bsky.social, @lgmartelotto.bsky.social, Anna Nam, and colleagues: bsky.app/profile/mgri... 7/n
Caleb Lareau
Capturing somatic mutations in single-cell probe-based assays (ie. 10X Flex) is a hard task, especially if you don't know *exactly* the mutations you want to capture, or if your target sequence is a mutational hotspot with many possible alternative alleles. Here's the way we pulled this off. Enjoy!
Marco Grillo
We GoT a Fillingโฆuuuuhoooo
๐ง๐ฎ๐ฟ๐ด๐ฒ๐๐ฒ๐ฑ ๐๐ฒ๐พ๐๐ฒ๐ป๐ฐ๐ถ๐ป๐ด ๐ผ๐ณ ๐บ๐๐๐ฎ๐๐ถ๐ผ๐ป๐ ๐๐ถ๐ฎ ๐ฅ๐ก๐-๐๐ฒ๐บ๐ฝ๐น๐ฎ๐๐ฒ๐ฑ ๐ด๐ฎ๐ฝ ๐ณ๐ถ๐น๐น๐ถ๐ป๐ด ๐ผ๐ณ ๐ผ๐น๐ถ๐ด๐ผ๐ป๐๐ฐ๐น๐ฒ๐ผ๐๐ถ๐ฑ๐ฒ๐ ๐ณ๐ผ๐ฟ ๐๐ถ๐ป๐ด๐น๐ฒ-๐ฐ๐ฒ๐น๐น ๐ฅ๐ก๐-๐๐ฒ๐พ
www.biorxiv.org/content/10.6...
