Online Now: Structure and engineering of the large serine recombinase Bxb1 for gene integration Online now:
Soma et al. present cryo-EM structures of the large serine recombinase Bxb1 bound to attP and attB substrates and provide mechanistic insights into attP/attB recognition, synaptic complex formation, and strand exchange via subunit rotation, enabling structure-guided engineering of Bxb1 variants with reprogrammed DNA preferences and enhanced recombination activity.
