Chromatin architectures underlying plasmid-based assays for regulatory variant effects
Mallory et al. develop plasmid Fiber-seq to map chromatin architecture along individual transfected plasmids at near-nucleotide resolution, which reveals that plasmids adopt heterogeneous chromatin patterns that regulate transcriptional activity. Combined with high-throughput reporter assays, this approach distinguishes the molecular mechanisms by which non-coding variants alter regulatory element activity.
