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This surprisingly relaxing footage is from SIX MILES under the ocean – and it’s the deepest ecosystem yet discovered
10mo
Now finally published as a Version of Record: In @elife.bsky.social: Membrane binding controls the ATPase cycle and localization of MinD in Bacillus subtilis doi.org/10.7554/eLif...
4d
Video
Delighted to see the main work from my PhD finally published in @mbio.bsky.social! It all started with the observation that deleting the cutRS two-component system in S. venezuelae caused this amazing explorer phenotype in the presence of glucose. But what was going on?! (1/n)
Dynamic relocalization of MinD in Bacillus subtilis arises from membrane binding of monomers and dimers with membrane-triggered ATP hydrolysis, suggesting MinE-like activators are unnecessary for Min ...
doi.org
Membrane binding controls the ATPase cycle and localization of MinD in Bacillus subtilis
🚨🔬🤖🚀 #PhotoFiTT is out! With @guijacquemet.bsky.social's & @microbematos.bsky.social's labs, we launched a new phototoxicity measurement for live microscopy using cell clocks⏱️. By rockstars @mariodelr.bsky.social @gomez-mariscal.bsky.social et al! AI-powered + label-free doi.org/10.1038/s414...
Helge Feddersen
Nature
5mo
6mo
Cool Paper: www.nature.com/articles/s41... Describing a molecular mechanism for persister formation in B. subtilis
May 16, 2025
New Grace Biolabs substitutes for GeneFrames look very promising. Will test soon
EarlyBird & Talk Submission Deadline for our Prokaryotic Cell Biology Conference is just around the corner, dont leave it too late to register and submit your abstract! Register or send your abstract to [email protected] before 12th January for consideration! ➡️https://bit.ly/4plmKtr
Fellow bacterial cell biologists, message me if you use @thermofishersci.bsky.social Gene Frames for microscopy! Thermo are planning to discontinue these in September but have indicated they will look into this further. If I make list of labs using them Thermo might not kill the product Please RT🔬🦠
Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.
May 12, 2025
6mo
Alarmone synthesis depletes GTP levels leading to a GTP-dependent switch that controls triggered, spontaneous and antibiotic-induced persister formation in Bacillus subtilis.
www.nature.com
A shared alarmone–GTP switch controls persister formation in bacteria - Nature Microbiology
Apr 8, 2025
11mo
Ricardo Henriques
Helge Feddersen
Tom McLean
42.1K Views. University of Groningen. This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy....
www.jove.com
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
Fusion Conferences
It’s FINALLY out! 🎉🚀 We have been working on PhotoFiTT for a long time, and it’s now published, introducing a quantitative framework to measure phototoxicity and optimise live-cell microscopy experiments. So excited to finally share the last version! 🔬✨ #PhotoFiTT doi.org/10.1038/s414...
Séamus Holden 🔬🦠🧫
Séamus Holden 🔬🦠🧫
Kai Johnsson
6mo
Mario Del Rosario