Develops single-molecule imaging tools to gain detailed information about cellular nanoscale structures, dynamics, and molecular mechanisms.
https://gustavssonlab.rice.edu
Anna-Karin Gustavsson
This week we were honored to host the Crown Princess of Sweden for a royal visit in our lab!
www.kungahuset.se/arkiv/nyhete...
www.townandcountrymag.com/society/trad...
Anna-Karin Gustavsson
Wednesday, 10:30 am: Poster B85 - Henry Gatica-Gutierrez, Quantitative super-resolution microscopy of DNA damage and repair for mechanistic insight into the FLASH effect in radiation therapy
Monday, 1:45 pm: Poster B396 - Siyang Cheng, Microfluidics-assisted single-objective lattice light sheet microscopy for enhanced single-molecule imaging in cells
Saturday, 2:25 pm: Biological Fluorescence Subgroup Symposium, room 303/304 – Anna-Karin Gustavsson, Multiplexed whole-cell single-molecule super-resolution imaging in 3D
Excited to be on my way to #BPS2026! Please check out the following presentations if you are there and want to learn more about our work. Also let me know if you want to meet and catch up!
Saturday, 10:25 am: Single-Molecule Forces, Manipulation, and Visualization Subgroup Symposium, room 153 – Siyang Cheng, Microfluidics-assisted single-objective lattice light sheet microscopy for enhanced single-molecule imaging in cells
Wednesday, 10:30 am: Poster B390 - Nahima Saliba, Adaptable and scalable reflective micromirrors for single-objective light sheet imaging in microfluidic and conventional chambers
Sunday, 1:45 pm: Poster B167 - Yael Shalev Ezra, High-throughput, high-resolution visualization of DNA repair in live Saccharomyces cerevisiae reveals chromatin reconfiguration following SSBs and DSBs
Excited that our work on versatile and scalable micromirror inserts that enable high-contrast and high-resolution single-objective light sheet microscopy in conventional sample chambers is now published in Nano Letters!
doi.org/10.1021/acs....
@pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale
We present a tunable microfabrication pipeline for creating robust, reflective inserts that adapt conventional commercial imaging chambers for single-objective light sheet (LS) illumination. This system reduces the complexity associated with dual-objective LS setups and specialized LS chambers while retaining the native functionality and biocompatibility of the original chambers. The fabricated insert features a metalized, 3D nanoprinted micromirror with an angled reflective surface, enabling alignment of a thin LS for sectioning and imaging throughout mammalian cells. Using this pipeline, we demonstrate that single-objective LS illumination achieves an over 4X improvement in the signal-to-background ratio compared with conventional widefield epi-illumination in both fixed and live cell samples. Furthermore, we show substantial resolution enhancement for single-molecule localization microscopy compared to epi-illumination for improved imaging at the nanoscale. The versatile and scalable design offers an easily implemented approach to bring the benefits of single-objective LS microscopy to a wide array of biological studies.
